Résumé : "Next Generation Sequencing" represents a class of relatively new technologies (first results published in 2007) which enable sequencing millions of short nucleotide sequences (reads) in several days. I will briefly discuss several biological applications of "Next Generation Sequencing", such as ChIP-Seq, transcriptome sequencing, small-RNA sequencing, whole genome sequencing. I will summarize the main challenges of the primary steps data analysis: assembly or alignment of reads, normalization of read counts. If I have time I will also talk about "Next-Next Generation Sequencing" (first results published in 2010).
Références :

• http://www.raindancetechnologies.com/documents/Next-Generation-Sequencing_Nature-Genetics_Jan-2010.pdf Michael L. Metzker (2010) Sequencing technologies : the next generation. Nature Review Genetics
• http://fr.wikipedia.org/wiki/Séquençage_de_l'ADN

 [Slides.pdf]

Dernière modification : Thursday 21 November 2024

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